Comparison of the Buffer Range Between MOPS and Other Buffers

The Good's Buffer is a series of N-substituted sulfamic acids that have good pH stability and are inert to a variety of chemicals and enzymes. 3-(N-morpholino)propanesulfonic acid, referred to as MOPS, is one of Good's Buffer, it plays a very important role in the biological experiments.

What are specific experiments MOPS used in?

The buffer range of MOPS is between 6.5 and 7.9, which is

(1) Suitable for the study of electron transfer and phosphorylation of the chloroplast thin layer;
(2) Can be prepared into a variety of agar medium and used as a non-toxic buffer in Streptomyces culture and cephalosporin production, and Lysis buffer that can be used for Escherichia coli cells;
(3) Can be used as electrolyte system components for isoelectric focusing electrophoresis (IEF) of two-dimensional gel electrophoresis;
(4) Can be used in Northern hybrid, as RNA separation and membrane buffer;
(5) Can be used for bicinchoninic acid (BCA) assay.

Comparison of the buffer range between MOPS (CAS 1132-61-2) and other buffers:

Table 1. The buffering range of MOPS and other buffers.

How to prepare the MOPS buffer?

The preparation method of commonly used 10× MOPS buffer is as follows:

1. Add 41.8g MOPS in 1L beaker;

2. Add about 700mL DEPC to stir and dissolve MOPS;

3. Use 2N NaOH to adjust the pH to 7.0;

4. Add the following reagents to the solution: 20mL of 1M NaOAc (DEPC treated), 20mL of 0.5M EDTA (pH 8.0) (DEPC treated), EDTA has a good complexation effect for the divalent, trivalent metal cations, and can effectively reduce the concentration of free metal cation ion. It also can make the pH stable; EDTA can also be used to suspend some of the enzymatic reaction;

5. Set the solution to 1L with DEPC treated water;

6. Remove impurities with 0.45μm filter;

7. Store at room temperature.

MOPS buffer is a zwitterion buffer, if the usage amount is small, it should be properly split charging. For safety and health, experimenter should wear clothing and disposable glove. If accidentally contact with the eyes, the operator should immediately rinse with plenty of water and seek medical attention.

Edited by Suzhou Yacoo Science Co., Ltd.

Related Reading: 3 n morpholino propanesulfonic acid buffer,high purity 3 n morpholino propanesulfonic acid