2018年9月21日星期五

HEPES and PIPES buffer, what differentiates them?

In biochemical experiments, buffer solution plays an indispensable role, it can resist the influence of a small amount of strong acid and alkali and maintain the pH value closest to the physiological environment for the system. HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid) buffer and PIPES buffer are both commonly used in biological experiments, both of which are Good's buffers and have similar structures. Many people often have doubts: Besides the structure, what is the difference between them?

Similarities between HEPES and PIPES buffers

HEPES and PIPES buffers, even all Good's buffers, have the following characteristics:
(1) pKa value between 6.0 and 8.0;
(2) High solubility in water;
(3) Membrane impermeability and not easy to penetrate biofilm;
(4) Limited impact on biochemical reactions, chemical and enzymatic hydrolysis, and no complex or precipitation with metal ions;
(5) Very low absorption of visible light and ultraviolet light;
(6) Ion concentration, solution composition and temperature have little effect on dissociation;
(7) Not participate or interfere with biochemical processes

What is the difference between them?


In summary, both HEPES buffer (CAS 7365-45-9) and PIPES are Good’s buffers, which do not form stable complexes with metal ions and are suitable for solution systems containing metal ions. However, there is also a certain difference between them. Therefore, when selecting the above buffer, we need to comprehensively consider the suitability of the experimental system and the difference in the nature of the two.


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